OPAL Optical O2 Measurement System Key Research Applications: Absolute Oxygen Concentration in Cell Cultures or Tissue, In-Vitro Hypoxia conditions in 2D or 3D Cell Culture (Tumor Models or Metabolism Studies), Measurement of Oxygen Conusmption or Production, Extracellular and Intracellular Oxygen in Cells, Tissues and Spheroids and Live Cell Imaging Assays.
OPAL Optical O2 Measurement System
The OPAL System allows for quantitative, real-time monitoring of absolute oxygen values in both the extracellular and intracellular environments of cells, tissue and spheroids. The system uses phosphorescence lifetime to measure the concentration of oxygen by way of oxygen sensitive fluorophores in the form of cell-impermeable nanoparticlse.
During live-cell imaging assays, a stage-top incubation system is used to control the oxygen and carbon dioxide levels; however, oxygen concentration is typically lower near or in cell clusters than in the incubation chamber. Knowledge of the precise oxygen levels in or near an experimental sample is often imperative for an accurate understanding of cellular activity.
Key Features: Non-Invasive Real Time Measurement, Fast Measurement within Seconds, High Spatial Resolution, Easily Connected to your Inverted Microscope, Compatible with Inverted Fluorescence Microscopes from Nikon, Olympus, Leica and Zeiss.
The System Includes: Controller, Detector Unit, LED Ligth Source, Adapter Set, Filter Cube and PC Software.