VHP Biodecontamination System for your Enclosed Workspaces

Compact, hands-free system, easy to use and reliable results

MycoFog’s Biodecontamination System is designed to eliminate microbiological contaminants—including bacteria, viral contaminants and fungi — from enclosed laboratory workspaces, such as hypoxia incubated workstations, anaerobic and microaerophilic workstations, chambers and incubators. This system creates the hydrogen peroxide fog (vapor or vH₂O₂) that biodecontaminates your enclosed workspace. Protect your bacterial, plant, tissue and cell cultures from all biological contaminants with our fast, effective, and easy-to-use MycoFog biodecontamination system

MycoFog Biodecontamination System

MycoFog BioDecontamination Unit MF-2D

Watch MycoFog Video below
Open MycoFog MF-2D User Manual
Open MycoFog MF-500D User Manual

MycoFog Key Features and Benefits

Protect Your Cell Cultures from Biological Contaminants
Fast and Easy to Use
Safe and Non Toxic
Ready to Use Consumables
Through Decontamination
Works under Low Temperature
Options for Workstations up to 1200L
Low Cost

Decontaminates your incubator in just 180 minutes.

Simply pour in the reagent, place the instrument inside your incubator, press start and walk away.

Uses a low temperature hydrogen peroxide fog that is safe for lab equipment and leaves no residual.

Pre-packed, ready-to-use recyclable bottles containing a proprietary, light- and air-sealed formulation.

Mycofog works under low temperature 28 -50˚C, working best at 45˚C.

Options for any incubator or workstation sizing up to 1200L. Contact us at info@scipro.com for more options.

A cost-effective solution for preventing and eliminating incubator and workstation contamination.

MycoFog BioDecontamination System MF-2D Unit

MycoFog ® FAQs & Troubleshooting Guide

Explore the list of Frequently Asked Questions listed below or contact us at info@scipro.com with any questions.

You can also Open MycoFog FAQ & Troubleshooting Guide Here

Where can I purchase MycoFog® and MycoFog® reagents?

SciPro is an official Distributor for MycoFog in the US and in Canada
You can buy the Units and all the Accessories from us.

US/Canada – Contact Details
Visit our Online Store at www.scipro.com
Email us at info@scipro.com
Call us at 1-800-268-1956

Need More Help?
Contact us at info@scipro.com

What is MycoFog®?

MycoFog® is an advanced biodecontamination system that uses hydrogen peroxide
(H₂O₂) fogging to eliminate microbial contamination from incubators, workstations, and
other enclosed spaces.

What reagents can I use with MycoFog®?

MycoFog® H₂O₂ reagents (our approved reagent for optimal performance). See below:

Order Part Number MFR-1Bx-K – MycoFog® reagent, 4 bottles + 4 wicks. For Incubators / Workstations <200L
Order Part Number MFR-2Bx-K – MycoFog® reagent, 4 bottles + 4 wicks. For Incubators / Workstations<350L
Order Part Number MFR-3Bx-K – MycoFog® reagent, 2 bottles + 2 wicks. For Incubators / Workstations>350L and <500L
Order Part Number MFR-4Bx-K – MycoFog® reagent, 2 bottles + 2 wicks. For Incubators / Workstations>500L and <700L
Order Part Number MFR-5Bx-K – MycoFog® reagent, 2 bottles + 2 wicks. For Incubators / Workstations>700L and <1000L
Order Part Number MFR-6Bx-K – MycoFog® reagent, 2 bottles + 2 wicks. For Incubators / Workstations>1000L and <1200L

Other compatible H₂O₂ solutions can be used (but please ensure proper concentration and purity). Please note that MycoFog® has not validated other H2O2 formulations for use with the MycoFog® instrument, so at this time we do not have supporting data except for the MycoFog® reagent.

How often should I perform a MycoFog® decontamination cycle?

For optimal contamination control, we recommend performing a MycoFog® cycle once per month per incubator.

How do I prepare MycoFog® for a decontamination cycle?

A. Ensure your Incubator is clean! The MycoFog® biodecontamination process complements cleaning but does not replace cleaning. Please consult your incubator operating manual for the manufacturer’s cleaning protocol
B. Ensure the incubator is empty (remove cultures, labware, and water pans).
C. Set the temperature to 45°C (or use the 37°C protocol for 4 hours).
D. Disable gas control (CO₂ and humidity settings).
E. Place MycoFog® at mid-level inside the incubator for optimal fog distribution.
F. Press the function button to start the cycle.

How long does the MycoFog® cycle take?

The Standard Cycle is 180 minutes at 45°C
The 37°C Protocol is 4 hours for <200L chambers, 5 hours for larger chambers

Can I use MycoFog® in large incubators?

Yes! There are 2 Instrument models of MycoFog® to choose from:

Model MF-2D – for Incubators / Workstations up to <500L internal volume
Model MF-500D – for Incubators / Workstation >500L but <1200L internal volume

What MycoFog Products should I Order?

MycoFog® Biodeconatmination System comes in a few different configurations. The Unit or Instrument itself, Reagent Kits and Starter Kits all designed for a particular size of Incubator/Glovebox/Workstation:

Order the correct MycoFog® Starter Kit and/or Reagent Pack for your Instrument (see Table Below)

Choose the right MycoFog® products and Reagents
Incubator or Workstation to be decontaminated by Internal Vol (L)
Internal Volume <200L – Order MycoFog Unit MF-2D – Reagent Kit MF-1Bx-K – Starter Kit MF-2D-SK-1Bx
Internal Volume >200L<350L – Order MycoFog Unit MF-2D – Reagent Kit MF-2Bx-K – Starter Kit MF-2D-SK-2Bx
Internal Volume >350 <500L – Order MycoFog Unit MF-2D – Reagent Kit MF-3Bx-K – Starter Kit MF-2D-SK-3Bx
Internal Volume >500 <700L – Order MycoFog Unit MF-500D – Reagent Kit MFR-4Bx-K – Starter Kit MF-500D-SK-4Bx
Internal Volume >700 <1000L – Order MycoFog Unit MF-500D – Reagent Kit MFR-5Bx-K – Starter Kit MF-500D-SK-5Bx
Internal Volume >1000 <1200L – Order MycoFog Unit MF-500D – Reagent Kit MFR-6Bx-K – Starter Kit MF-500D-SK-6Bx

What is the recommended relative humidity before starting a cycle?

MycoFog® works best when the incubator’s starting relative humidity is ambient.
The device achieves proper humidity during the cycle.

Can I use MycoFog® in an incubator that contains powered electronics?

Yes. MycoFog® has been tested with electronic devices such as the Etaluma
Lumascope and the Sartorius Incucyte which perform normally after repeated
decontamination cycles.

MycoFog® Instruments now come with a delayed start function?

The new D-series MycoFog® instruments (MF-2D and MF-500D) include the following two key
safety enhancements:

Press + Hold to Initiate: Instead of a short press, the decontamination cycle now
requires a 1.5-second hold on the function button. This reduces the chance of
inadvertently starting vaporization.

12-Second Delayed Start: After initiating the cycle, the instrument waits 12
seconds before H₂O₂ vaporization begins. The indicator light flashes slowly for 7
seconds, then rapidly for 5 seconds, and only then does fogging start. This gives users
extra time to close both inner and outer incubator doors and reduces any risk of
exposure to hydrogen peroxide vapor at the start of the cycle.

Troubleshooting – My MycoFog® instrument is not producing enough spray

What Should I Do?
A. Check the Wick – Remove the wick.
Ensure it is fully saturated and reseat wick.
Ensure the spring is in the wick holder and that the wick holder is seated properly.
B. Check the Reagent – Ensure the correct amount is added and that only MycoFog®
H₂O₂ reagent or an approved formulation is used.
C. Clean the Fogger – Use a cotton swab with vinegar to clean the nebulizer element.
D. Charge the Battery – Low power can reduce spray intensity.
Fully charge before use.

Troubleshooting – My MycoFog® stopped mid-cycle

How do I fix it?
A. Check the Battery – Battery may be low
Recharge the unit.
B. Restart the Unit – Restart the device by turning it off and on again.

Troubleshooting – The reagent is leaking—what should I check?

What should I check?
A. Check Reagent Reservoir – Ensure the reagent reservoir is properly sealed.
B. Check the Unit Level – Confirm that the instrument is level and not tilted.
C. Check the Reagent Volume – Do not overfill—use only the recommended reagent volume.

How long does the battery last?

A fully charged battery will perform for approximately 5 cycles.
We recommend fully charging the instrument before each use if repeated uses are done on different days

Can MycoFog® operate while charging?

Yes, MycoFog® can function while connected to a power source.

How do I clean my MycoFog® instrument?

After each use, rinse the reagent reservoir with distilled water.
If fogging volume decreases, clean the nebulizer disk with vinegar and a cotton swab.
Use a fresh wick for each cycle

What quality control checks are performed before shipping?

Each MycoFog® instrument is tested to ensure:
• Proper flow rate within the acceptable range
• Residual reagent volume is within specification

Can MycoFog® be used in a GMP environment?

Yes! Our solvent-resistant instrument labels allow for alcohol wipe-downs before GMP
suite entry

How do I validate MycoFog’s effectiveness?

Use an approved Biological Indicator (BI’s) to confirm a reduction in microbial contamination.
Use only MycoFog® BI’s or BI’s specifically made for ambient pressure environments.

MycoFog ® Specifications and Resources

Have a look at the Resources listed below or contact us at info@scipro.com for more information

Open MycoFog Unit Specifications
Open MycoFog Quick Start Guide
Open MycoFog Biodecontamination Flyer
Open MycoFog Reagents Safety Data Sheet
Open MycoFog Biological Indicators User Instructions
Open MycoFog Biodecontamination System Cleaning – White Paper
Open MycoFog Unit FAQ & Troubleshooting Guide

Contact us for more information.

VHP Biodecontamination System for your Enclosed Workspaces | MycoFog

Compact, hands-free system, easy to use and reliable results

MycoFog Biodecontamination System

MycoFog Key Features and Benefits

Read More

MycoFog Fogger Unit

MycoFog Starter Kit

MycoFog Prove it Yourself Kit

MycoFog Reagents

MycoFog Biological Indicators

MycoFog ® FAQs & Troubleshooting Guide

MycoFog ® Specifications and Resources